Aive antibody repertoire11/28/2023 ![]() ![]() To address this, PBMCs and plasma were collected from healthy controls, individuals immunized against SARS-CoV-2, or subjects with convalescent or severe SARS-CoV-2 infection. We asked whether SARS-CoV-2–associated inflammation impairs B ND cell peripheral tolerance. In vitro evidence suggests inflammatory stimuli can breach peripheral B cell tolerance in this subset. B ND cells are a B cell subset in healthy individuals harboring autoreactive but anergic B lymphocytes. Sblattero D, Bradbury A (1998) A definitive set of oligonucleotide primers for amplifying human V regions.Severe SARS-CoV-2 infection is associated with strong inflammation and autoantibody production against diverse self-antigens, suggesting a system-wide defect in B cell tolerance. Hoogenboom HR (2002) Overview of antibody phage-display technology and its applications. Griffiths AD, Malmqvist M, Marks JD, Bye JM, Embleton MJ, McCafferty J, Baier M, Holliger KP, Gorick BD, Hughes-Jones NC et al (1993) Human anti-self antibodies with high specificity from phage display libraries. ĭe Haard HJ, van Neer N, Reurs A, Hufton SE, Roovers RC, Henderikx P, de Bruine AP, Arends JW, Hoogenboom HR (1999) A large non-immunized human Fab fragment phage library that permits rapid isolation and kinetic analysis of high affinity antibodies. Vaughan TJ, Williams AJ, Pritchard K, Osbourn JK, Pope AR, Earnshaw JC, McCafferty J, Hodits RA, Wilton J, Johnson KS (1996) Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Human antibodies from V-gene libraries displayed on phage. Marks JD, Hoogenboom HR, Bonnert TP, McCafferty J, Griffiths AD, Winter G (1991) By-passing immunization. Pelat T, Hust M, Thullier P (2009) Obtention and engineering of non-human primate (NHP) antibodies for therapeutics. Īyat H, Burrone OR, Sadghizadeh M, Jahanzad E, Rastgou N, Moghadasi S, Arbabi M (2013) Isolation of scFv antibody fragments against HER2 and CEA tumor antigens from combinatorial antibody libraries derived from cancer patients. Kim SJ, Jang MH, Stapleton JT, Yoon SO, Kim KS, Jeon ES, Hong HJ (2004) Neutralizing human monoclonal antibodies to hepatitis A virus recovered by phage display. Pasello M, Zamboni S, Mallano A, Flego M, Picci P, Cianfriglia M, Scotlandi K (2016) Design and construction of a new human naive single-chain fragment variable antibody library, IORISS1. Weber M, Bujak E, Putelli A, Villa A, Matasci M, Gualandi L, Hemmerle T, Wulhfard S, Neri D (2014) A highly functional synthetic phage display library containing over 40 billion human antibody clones. įrenzel A, Kugler J, Wilke S, Schirrmann T, Hust M (2014) Construction of human antibody gene libraries and selection of antibodies by phage display. Silacci M, Brack S, Schirru G, Marlind J, Ettorre A, Merlo A, Viti F, Neri D (2005) Design, construction, and characterization of a large synthetic human antibody phage display library. Human antibodies with subnanomolar affinity against a marker of angiogenesis eluted from a two-dimensional gel. ![]() Pini A, Viti F, Santucci A, Carnemolla B, Zardi L, Neri P, Neri D (1998) Design and use of a phage display library. Hust M, Frenzel A, Meyer T, Schirrmann T, Dubel S (2012) Construction of human naive antibody gene libraries. ![]()
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